Cre translocation
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Cre translocation
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Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented … See more Cre-Lox recombination is a special type of site-specific recombination developed by Dr. Brian Sauer and patented by DuPont that operated in both mitotic and non-mitotic cells, and was initially used in activating gene … See more During genetic recombination, a Holliday junction is formed between the two strands of DNA and a double-stranded break in a DNA molecule … See more The P1 phage is a temperate phage that causes either a lysogenic or lytic cycle when it infects a bacterium. In its lytic state, once its viral genome is injected into the host cell, viral … See more • Introduction to Cre-lox technology by the "Jackson Laboratory" See more Cre-Lox recombination involves the targeting of a specific sequence of DNA and splicing it with the help of an enzyme called Cre recombinase. Cre-Lox recombination is … See more Site-specific recombination (SSR) involves specific sites for the catalyzing action of special enzymes called recombinases. Cre, or cyclic … See more A classical strategy for generating gene deletion variants is based on double cross-integration of non-replicating vectors into the genome. … See more WebCre-mediated possibilities for site specific (and often cell type specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed.
WebOct 5, 2024 · Insulin stimulates glucose uptake in adipose tissue and skeletal muscle by inducing plasma membrane translocation of the glucose transporter GLUT4. Although the small GTPase Rac1 is a key regulator downstream of phosphoinositide 3-kinase (PI3K) and the protein kinase Akt2 in skeletal muscle, it remains unclear whether Rac1 also …
WebThe Cre-Lox recombination system is derived from P1 Bacteriophages and uses a specific recombinase protein (Cre) that targets a unique DNA sequence (loxP) for splicing. Flanking a DNA sequence with loxP sites … WebJul 23, 2015 · A mouse model that mediates temporal, specific, and efficient myocardial deletion with Cre-LoxP technology will be a valuable tool to determine the function of genes during heart formation. Mhy6 encodes a cardiac muscle specific protein: alpha-myosin heavy chain. Here, we generated a new Myh6-MerCreMer (Myh6MerCreMer/+) inducible …
Web283 rows · Translocation: If the sites are on separate DNA molecules, a …
WebJan 24, 2024 · In breast cancer, ECM1 is essential for the ability of breast cancer cells to undergo matrix attachment, invasion, and metastasis by promoting mosin membrane translocation and phosphorylation . In gastric cancer, ECM1 can regulate gastric cancer cell metastasis and glucose metabolism by inducing the ITGB4/FAK/SOX2/HIF-1α signal … heart rate monitor watch strapWebJun 15, 2024 · Here, we show that cellular redox homeostasis maintained by glutathione peroxidase 4 (GPX4) is required for STING activation. GPX4 deficiency enhanced cellular lipid peroxidation and thus ... heart rate monitor waterproofWebFeb 3, 2024 · Crosses with gfp-stacked lines (i.e., 325-I and 537-I) leads to Cre-mediated reciprocal translocation of the lox-flanked DNA. (L,M) PCR results from deletion and … heart rate monitor weight watchers