Flp frt 重组系统与 mosaic 杂交实验
WebMay 1, 1998 · Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label ... WebCatalog number: K601001. Our Flp-In™ Complete System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice.
Flp frt 重组系统与 mosaic 杂交实验
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WebSep 20, 2013 · We describe here the Tomato/GFP-FLP/FRT method, which can be used for rapid clonal analysis in the eye of living adult Drosophila. Fluorescent photoreceptor cells are imaged with the cornea neutralization technique, on retinas with mosaic clones generated by flipase-mediated recombination. This method has several major … WebJun 25, 2013 · 条件性基因敲除的基本原理Flp/FRT系统 该系统与Cre/loxP系统相同,也是由一个重组酶和一段特殊的DNA序列组成。从进化的角度上考虑,Flp/FRT系统 …
Web实验七 利用FLP/FRT 重组系统获得纯合突变克隆,Mosaic杂交实验. 介绍基因功能研究策略的背景知识,以被誉为果蝇遗传学家瑞士军刀的Gal4-UAS系统原理和灵活多样的使用为 … WebMay 28, 1998 · A cassette was constructed that contains a GmR selectable marker next to the green fluorescent protein structural gene, with both markers being flanked by Flp recombinase target (FRT) sites. The FRT cassette was used to insertionally inactivate the cloned P. aeruginosa pabC gene encoding aminodeoxychorismate lyase.
WebDec 15, 2012 · Combination of the FLP/FRT technology with the UAS/GAL4 system allows for the control and visualization of gene expression in mosaic clones or for killing off wild-type cells such that only mutant cells contribute to the final organ or tissue of interest (Brand and Perrimon, 1993; Chou and Perrimon, 1996; Stowers and Schwarz, 1999; Pagliarini ... WebOct 25, 2010 · flp 是一个来自酵母的染色体重组酶,而 frt 则是 flp 催化的一段 dna 序列。 如果把 FLP 和 FRT 通过转基因导入果蝇基因组中,在 FLP 的催化下,含有 FRT 序列的 …
WebThe FLP/FRT-based genetic mosaic system depends on FLP-mediated mitotic recombination between FRTs residing in trans on homologous chromosomes 21,22,23. …
WebFlp-FRT系统与Cre-loxP诱导基因重组的方式类似,这两个系统比较明显的区别是这个重组酶(Cre和Flp)具有不同的最佳反应温度,有研究发现,Cre重组酶的最佳温度为37℃, … gazeta de alagoas fmWebWe have modified an FLP/FRT-based genetic mosaic system to label either neurons derived from a common progenitor or isolated single neurons, in the Drosophila CNS. These uniquely labeled neurons can also be made homozygous for a mutation of interest within an otherwise phenotypically wild-type brain. Using this new mosaic system, not only can ... gazeta cyWebApr 1, 1998 · Abstract. Marked clones in mosaic animals have been used extensively to answer developmental questions in Drosophila. Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label mutant clones in both … auto mieten japan kosten