WebApr 5, 2024 · This method is useful for both regular untargeted metabolomics and stable isotope tracing experiments. It is implemented as a Python package (khipu) and provides a JSON format for easy data ... WebSep 25, 2012 · The combination of high-resolution LC–MS-based untargeted metabolomics with stable isotope tracing provides a global overview of the cellular fate of precursor …
Global stable-isotope tracing metabolomics reveals system …
WebSep 23, 2024 · Stable isotope-resolved metabolomics (SIRM) provides information regarding the relative activity of numerous metabolic pathways and the contribution of nutrients to specific metabolite pools ... WebNational Center for Biotechnology Information twins at wdia
Metabolomics, metabolic flux analysis and cancer pharmacology
WebNov 11, 2024 · Figure 1.Steps in stable-isotope metabolomics analysis. Stable-isotope tracers to study cardiac metabolism are administered to the model organism or patient using different delivery approaches including, infusion, injections, diet, or ex vivo perfusion. Heart tissue or biofluids are collected and metabolites are extracted based on downstream … WebFeb 27, 2024 · A stable-isotope tracing capillary electrophoresis–mass spectrometry (CE-MS) metabolomic approach was developed to cover both polar metabolites and isotopologues in a non-targeted way. Abstract Mass spectrometry (MS) driven metabolomics is a frequently used tool in various areas of life sciences; however, the … WebJun 20, 2024 · We conducted the continuous stable-isotope labeling in Drosophila using [U-13 C]-glucose and collected head tissues at six time intervals for global isotope tracing metabolomics. In head tissues, a total of 745 metabolites were annotated, and 390 labeled metabolites from 59 metabolic pathways were tracked by MetTracer (Fig. 2a and … twins at the tipton cast