NettetHow to calculate Vmax and Km from a Lineweaver-Burk plot. Nigel Francis. 334 subscribers. Subscribe. 47K views 3 years ago. A step by step guide for calculating … Nettet13. nov. 2024 · The Lineweaver-Burk plot is also useful when determining the type of enzyme inhibition present by, comparing its effect on Km and Vmax. Fig 3 – Different types of enzyme inhibition as shown on a Lineweaver-Burk plot Clinical Relevance - Plasma Enzyme Assays Plasma enzyme assays can detect abnormal levels of …
Application of modified Michaelis – Menten equations for determination ...
NettetDisplay a plot of the above data in Michaelis-Menton form (v0 vs [5]), being sure to show the numerical values (and units) of VmaX and KM on the plot. d. Display a plot of the … NettetThe reciprocal values of the data are plotted in order to follow the Lineweaver Burke equation and transform the plotted points from a curve to a line. In this form the slope and intercepts are easier to identify. The slope of a Lineweaver-Burke plot is equivalent to Km/Vmax, the x-intercept -1/Km, and the y-intercept is 1/Vmax. high tides smoke shop
Lineweaver–Burk plot - Wikipedia
NettetI suggest you use non-linear regression to determine your Km and Vmax. This can be done in GraphPad Prism, for instance. If you don't have the software, there are a bunch … NettetLineweaver-Burk Plot. Lineweaver Burk plots show that the Vmax was calculated at 9 nmoles per mg per 30 min, or 1.3 nmoles per pineal per 30 min. From: Serotonin and … NettetQuestion 8: (20 points) Using the following data set, graph a primary plot, a lineweaver burk plot and calculate KM and Vmax (round to the nearest tenth) for the enzyme. Also determine what type of inhibitor each inhibitor is and provide a lineweaver burk plot that shows the enzyme and its inhibited rate. high tides seafood restaurant bend oregon