Smad4 sc-7966
Webb24 aug. 2013 · All incubations with anti-SMAD4 antibody (B-8, sc-7966, Santa Cruz, USA) at 1:200 dilutions were carried out overnight at 4 °C. Then, the specimens were briefly washed in PBS (3×5 min) and incubated at room temperature with anti-rabbit antibody and then streptavidin–biotin protein peroxidase (Maixin Fuzhou, China). WebbDownload scientific diagram Smad3 and Smad4 cooverexpression represses endogenous c- myc expression. (A) BALB/MK cells either were treated with 400 nM TGF-  or vehicle for 60 min or were ...
Smad4 sc-7966
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Webb1 okt. 2010 · Paraffin sections (7.5 μm thick) were incubated with the following primary antibodies: Anti-Smad4 (sc-7966, Santa Cruz Biotechnology, CA), Rabbit polyclonal anti-alpha and anti-beta Crystallin antibodies provided by Dr. Samuel Zigler (John Hopkins University, MD) for 1 h at 37 °C following antigen retrieval and blocking. WebbAlthough cytoplasmic AMP-activated protein kinase (AMPK) has been known as a tumor-suppressor protein, nuclear AMPK is suggested to support clear cell renal cell carcinoma …
Webb2 juni 2011 · Mouse anti-Smad4 (1:300, clone Sc-7966; Santa Cruz Biotechnology) and rabbit anti-Hoxa9 (1:300, clone 07-178; Upstate Biotechnology) antibodies were applied … Webb27 aug. 2012 · In situ proximity ligation assays showed that TGFβ signaling not only induces complexes between Smad3 and Smad4 in the nucleus but also complexes between Smad2/3 and Fra1, whereas complexes...
Webb14 maj 2010 · The chromatin solution was subjected to immunoprecipitation using a OneDay ChIP Kit (Diagenode, Sparta, NJ) according to the manufacturer's instructions. … Webb14 mars 2014 · SMAD4 is a gastrointestinal malignancy-specific tumor suppressor gene found mutated in one third of colorectal cancer specimens and half of pancreatic …
Webb24 aug. 2013 · incubations with anti-SMAD4 antibody (B-8, sc-7966, Santa Cruz, USA) at 1:200 dilutions. were carried out overnight at 4 °C. Then, the specimens were briefly washed in PBS (3×5.
WebbSMAD4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue … ctr operationsWebbDownload scientific diagram Smad3 and Smad4 cooverexpression represses endogenous c- myc expression. (A) BALB/MK cells either were treated with 400 nM TGF-  or vehicle … ctr open book referencesWebband knocking down HBx while replenishing SMAD4 rescued HCC cell proliferation. Mechanically, we found that HBx regulates SMAD4 expression at the transcriptional level … ctropmedWebbSmad4 (B-8) is available conjugated to agarose (sc-7966 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-7966 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; to either … c. tropicalis treatmentWebb7 Table S7. Antibodies Rabbit anti Rabbit anti Mouse anti Antibodies Company Dilution Rabbit anti-Kras polyclonal antibody ab180772 Abcam 1:100 Rabbit anti-p16 monoclonal … earthway fertilizer spreaders for saleWebb23 jan. 2004 · Smad proteins play key roles in intracellular signaling of the transforming growth factor-β (TGF-β) superfamily. E1A, an adenoviral oncoprotein, is known to inhibit TGF-β-induced transactivation through binding to Smad proteins. earthway fertilizer spreaders for lawnsWebb25 maj 2024 · SMAD4, a key regulator of transforming growth factor-β (TGF-β) signaling, plays a major role in cell growth, migration, and apoptosis. In particular, TGF-β/SMAD … c. tropicalis infection